Micropropagation of apple (Malus domestica L. cultivar Douce de Djerba) through in vitro culture of axillary buds

Contenu

Titre
Micropropagation of apple (Malus domestica L. cultivar Douce de Djerba) through in vitro culture of axillary buds
Date
2010
Dans
Acta Botanica Gallica
Résumé
A high frequency of sprouting (85.0%) and shoot differentiation is observed in the primary cultures of nodal explants of Malus domestica L. cultivar Douce de Djerba on MS medium supplemented with BAP (1.0 mg.L−1) plus IBA (0.1 mg.L−1). In vitro proliferated shoots are multiplied rapidly by culture of shoot tips on MS medium with BAP (1.0 and 2.0 mg.L−1) which produce the greatest multiple shoot formation. The BAP has a positive effect on the multiplication and growth, but a concentration that exceeds 4.0 mg.L−1 decreases the growth. A high frequency of rooting (66.7%) with development of healthy roots is observed from shoots cultured on half strength MS medium enriched with IBA (3.0 mg.L−1) and 2.0 g.L−1 of activated charcoal. Plants with well developed roots were transferred to soil with a survival frequency of 60%.
Langue
eng
volume
157
numéro
3
pages
513-524
doi
10.1080/12538078.2010.10516227
issn
1253-8078
Source
Taylor and Francis+NEJM

Boudabous, Manel et al., “Micropropagation of apple (Malus domestica L. cultivar Douce de Djerba) through in vitro culture of axillary buds”, 2010, bibliographie, consulté le 21 décembre 2024, https://ibadica.org/s/bibliographie/item/13927

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