Micropropagation of apple (Malus domestica L. cultivar Douce de Djerba) through in vitro culture of axillary buds

Contenu

Boudabous, Manel, Mars, Mongia, Marzougui, Nidhal, et Ferchichi, Ali. 2009. « Micropropagation of Apple (Malus Domestica L. Cultivar Douce De Djerba) through in Vitro Culture of Axillary Buds ». Acta Botanica Gallica 157 (3): 513-24. doi:10.1080/12538078.2010.10516227, bibliographie, consulté le 3 avril 2025, https://ibadica.org/s/bibliographie/item/40296

Titre
Micropropagation of apple (Malus domestica L. cultivar Douce de Djerba) through in vitro culture of axillary buds
Sujet
Botanique -- Djerba
Résumé
A high frequency of sprouting (85.0%) and shoot differentiation is observed in the primary cultures of nodal explants of Malus domestica L. cultivar Douce de Djerba on MS medium supplemented with BAP (1.0 mg.L−1) plus IBA (0.1 mg.L−1). In vitro proliferated shoots are multiplied rapidly by culture of shoot tips on MS medium with BAP (1.0 and 2.0 mg.L−1) which produce the greatest multiple shoot formation. The BAP has a positive effect on the multiplication and growth, but a concentration that exceeds 4.0 mg.L−1 decreases the growth. A high frequency of rooting (66.7%) with development of healthy roots is observed from shoots cultured on half strength MS medium enriched with IBA (3.0 mg.L−1) and 2.0 g.L−1 of activated charcoal. Plants with well developed roots were transferred to soil with a survival frequency of 60%.
Est une partie de
Acta Botanica Gallica
volume
157
numéro
3
pages
513-524
Date
2010
Langue
eng
doi
10.1080/12538078.2010.10516227
issn
1253-8078

Boudabous, Manel, Mars, Mongia, Marzougui, Nidhal, et Ferchichi, Ali. 2009. « Micropropagation of Apple (Malus Domestica L. Cultivar Douce De Djerba) through in Vitro Culture of Axillary Buds ». Acta Botanica Gallica 157 (3): 513-24. doi:10.1080/12538078.2010.10516227, bibliographie, consulté le 3 avril 2025, https://ibadica.org/s/bibliographie/item/40296

Position : 23569 (18 vues)